HPLC Analysis of Drug Kynurenine on Heritage C18 Column

Conditions of Experiment
Column: Heritage C18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 250 mm, 5 um, 100A
Mobile Phase: ACN gradinet from 0% to 80% in 20 min
Detection: UV 225 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Class of compounds: Amino acid, Aromatic base
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Kynurenine