HPLC Analysis of Drug Loratadine and Related Impurities on Coresep SB Mixed-Mode Column

Application description

Loratadine is a non-sedating anti-allergy medication. It is available as a single component drug formulation or a composition with decongestant pseudoephedrine. Loratadine is a hydrophobic and slightly basic compound with a pKa around 5. Loratadine and related impurities were analyzed on the Coresep SB mixed-mode column. Compounds are retained based on reversed-phase and cation-exclusion mechanisms. The method is fast, robust and reproducible, not requiring ion-pairing reagents (IPC). Numerous mobile phases can be used with Coresep 100 column to accommodate various detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. The approach can be used for quantification of loratadine and related impurities in multiple matrices. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and cation-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 15% ACN with 0.2% of H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Aromatic base, Drug, Pyridine
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Loratadine, Related Impurities