HPLC Analysis of Drug Lorazepam and Related Impurities on Coresep 100 Mixed-Mode Column

Application description

Lorazepam is one of the benzodiazepine drugs which is used to treat anxiety disorders, sleeping issues, active seizures (including status epilepticus), alcohol withdrawals, and chemotherapy induced nausea. Aso, it can be used for surgery to interfere with memory formation and to sedate those who are being mechanically ventilated. Lorazepam is hydrophobic and neutral in nature. It is retained by reversed-phase mechanism on the Coresep 100 mixed-mode column. Ionizable impurities are retained and separated by reversed-phase, cation-exchange and anion-exclusion mechanisms. Various mobile phases can be used with Coresep 100 column to accommodate numerous detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 100A
Mobile Phase: MeCN 30% with 20 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Controlled substance, Drug
Nature of compounds: Hydrophobic, Neutral
Compounds: Lorazepam