HPLC Analysis of Drug Meclizine and Related Impurities on Coresep SB Mixed-Mode Column

Application description

Meclizine is a histamine H1 receptor antagonist which is used as a drug to treat nausea. It also serves as inhibitor of hepstin. It is used to treat motion sickness. Meclizine is a first generation antihistamine of piperazine class. It is hydrophobic and basic in nature. Poor peak shape is observed on traditional reversed-phase columns due to interaction of basic groups with residual silanols. This problem of poor peak shape was addressed by using Coresep SB mixed-mode column. Basic group on the surface of Coresep SB provides a shielding effect, and silanols do not interact with basic sites of the molecule. The compound retains on Coresep SB column by combination of reversed-phase and cation-exclusion mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of meclizine in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and cation-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 20% ACN with 0.2% of H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Meclizine, Related Impurities