HPLC Analysis of Drug Metoclopramide and Related Impurities on Coresep 100 Mixed-Mode Column

HPLC Analysis of Drug Metoclopramide and Related Impurities on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 65% ACN with 0.2% H3PO4
Detection: UV 235 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Analytes
1. Metoclopramide
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic

Application description

Metoclopramide is a drug used for treatment of stomach and esophageal problems. It is commonly used to treat and prevent nausea and vomiting. It is also used to treat migraine headaches. It is also used to treat the symptoms of gastroparesis. Metoclopramide is hydrophobic and basic in nature. It has two ionizable basic groups - aniline fragment and secondary amine. The drug and related impurities were separated on Coresep 100 HPLC column by combining reversed-phase and cation-exchange mechanisms. The method can be used for analysis of hydrophobic basic compounds in reversed-phase cation-exchange mode. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust, reproducible, and can be used for analysis of metoclopramide in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

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