HPLC Analysis of Drug Morphine and Related Impurities on Coresep 100 Mixed-Mode Column

HPLC Analysis of Drug Morphine and Related Impurities on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 45% ACN with 20 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 mL/min
Analytes
1. Morphine
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Controlled substance, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic

Application description

Morphine is used to relieve moderate to severe pain. It belongs to the group of medicines called narcotic analgesics (pain medicines). Morphine acts on the central nervous system (CNS) to relieve pain. When morphine is used for a long time it can cause addiction. Morphine and related impurities were analyzed by mixed-mode chromatography on Coresep core-shell multi-mode column. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. This method can be used in bioanalysis to quantify morphine in biofluids and drug formulations. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.

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