Application description
Morphine is used to relieve moderate to severe pain. It belongs to the group of medicines called narcotic analgesics (pain medicines). Morphine acts on the central nervous system (CNS) to relieve pain. When morphine is used for a long time it can cause addiction. Morphine and related impurities were analyzed by mixed-mode chromatography on Coresep core-shell multi-mode column. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. This method can be used in bioanalysis to quantify morphine in biofluids and drug formulations. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
45% ACN with 20 mM AmFm pH 3 |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.8 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic base, Controlled substance, Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic |
| Compounds: |
Morphine, Related Impurities |
