HPLC Analysis of Drug Ondansetron and Related Impurities on Coresep 100 Mixed-Mode Column

HPLC Analysis of Drug Ondansetron and Related Impurities on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 65% ACN with 0.2% H3PO4
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Analytes
1. Ondansetron
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic

Application description

Ondansetron is a drug used for treatment of nausea and vomiting caused by cancer chemotherapy and radiation therapy. Ondansetron is a serotonin 5-HT3 receptor antagonist. The drug is hydrophobic and slightly basic. Ondansetron and related impurities were separated on Coresep 100 column by combination of reversed-phase and cation-exchange mechanisms. The method is robust, reproducible, and can be used for analysis of ondansetron in formulation, biofluids, and other matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

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