HPLC Analysis of Drug Oxycodone on Coresep 100 Mixed-Mode Column
Application description
Oxycodone is opioid pain medication. It is Schedule II controlled substance. Oxycodone and its related impurities were analyzed on Coresep 100 column in reversed-phase cation-exchange mode. The retention time can be controlled by the amount of ACN, buffer pH and buffer concentration. This method is isocratic and compatible with MS detection. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | 60% ACN with 30 mM AmFm pH 3 |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Controlled substance, Drug |
Nature of compounds: | Basic, Hydrophobic |
Compounds: | Oxycodone |