HPLC Analysis of Drug Oxycodone on Coresep 100 Mixed-Mode Column

Application description

Oxycodone is opioid pain medication. It is Schedule II controlled substance. Oxycodone and its related impurities were analyzed on Coresep 100 column in reversed-phase cation-exchange mode. The retention time can be controlled by the amount of ACN, buffer pH and buffer concentration. This method is isocratic and compatible with MS detection. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 60% ACN with 30 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Controlled substance, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Oxycodone