HPLC Analysis of Drug Oxycodone on Coresep 100 Mixed-Mode Column
Conditions of Experiment |
Column: |
Coresep 100 |
Separation Modes: |
reversed-phase and cation-exchange |
Column Dimenstions: |
3.0 x 100 mm |
Mobile Phase: |
60% ACN with 30 mM AmFm pH 3 |
Detection: |
UV 275 nm |
Sample: |
0.3 mg/ml |
Injection: |
1 uL |
Flow rate: |
0.7 mL/min |
|
Analytes |
1. Oxycodone |
Class of compounds:
Controlled substance, Drug |
Nature of compounds:
Basic, Hydrophobic |
Application description
Oxycodone is opioid pain medication. It is Schedule II controlled substance. Oxycodone and its related impurities were analyzed on Coresep 100 column in reversed-phase cation-exchange mode. The retention time can be controlled by the amount of ACN, buffer pH and buffer concentration. This method is isocratic and compatible with MS detection. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.