HPLC Analysis of Drug Phentermine and Related Impurities on Coresep 100 Mixed-Mode Column

Application description

Phentermine is a drug with pharmacology similar to amphetamines. Phentermine is a sympathomimetic that releases norepinephrine and epinephrine in CNS and periphery reducing hunger. Similarly to other amphetamines, phentermine potently inhibits DA and 5-HT uptake. It is a psychostimulant drug which has Schedule IV classification in US. It is slightly hydrophobic in nature and has basic properties due to the presence of primary amine. HPLC analysis of phentermine and related impurities was performed on Coresep 100 mixed-mode column. Method is robust with good peak shape for main compound. Method can be used for analysis of hydrophobic basic compounds in reversed-phase cation-exchange mode. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is robust and reproducible and can be used for analysis of phentermine in formulation, biofluids, and other compositions. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 30% ACN with 0.1% TFA
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.5 mL/min
Analytes
Class of compounds: Aromatic acid, Aromatic base, Controlled substance, Drug, Stimulant
Nature of compounds: Basic, Hydrophilic, Hydrophobic
Compounds: Phentermine, Related Impurities