HPLC Analysis of Drug Promethazine on Heritage C18 Column

Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 250 mm, 5 um, 100A
Mobile Phase: 35% ACN with 20 mM NaH2PO4 pH 2.5
Detection: UV 254 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Promethazine