Application description
Fluoxetine (Prozac) is an antidepressant of the selective serotonin reuptake inhibitor(SSRI) class. It is used for the treatment of major depressive disorder, obsessive-compulsive disorder (OCD), bulimia nervosa, panic disorder and premenstrual dysphoric disorder. Fluoxetine is hydrophobic and basic in nature. Fluoxetine and related impurities were analyzed by mixed-mode chromatography on Heritage MA HPLC column. Compounds are separated by reversed-phase and cation-exclusion mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection).
Conditions of Experiment
| Column: |
Heritage MA |
| Separation Modes: |
reversed-phase and cation-exclusion |
| Column Dimenstions: |
4.6 x 150 mm |
| Mobile Phase: |
15% ACN with 0.2% H3PO4 |
| Detection: |
UV 215 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic base, Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic |
| Compounds: |
Prozac (fluoxetine), Related Impurities |
