HPLC Analysis of Drug Prozac and Related Impurities on Heritage MA Mixed-Mode Column

HPLC Analysis of Drug Prozac and Related Impurities on Heritage MA Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Heritage MA
Separation Modes:
reversed-phase and cation-exclusion
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 15% ACN with 0.2% H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Analytes
1. Prozac (fluoxetine)
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic

Application description

Fluoxetine (Prozac) is an antidepressant of the selective serotonin reuptake inhibitor(SSRI) class. It is used for the treatment of major depressive disorder, obsessive-compulsive disorder (OCD), bulimia nervosa, panic disorder and premenstrual dysphoric disorder. Fluoxetine is hydrophobic and basic in nature. Fluoxetine and related impurities were analyzed by mixed-mode chromatography on Heritage MA HPLC column. Compounds are separated by reversed-phase and cation-exclusion mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection).

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