HPLC Analysis of Drug Torbutrol on Coresep 100 Mixed-Mode Column
Application description
Torbutrol is a morphine type synthetic opioid analgesic. Torbutrol is used in migraine pain management. Compound is hydrophobic and basic in nature. Torbutrol was analyzed on Coresep 100 mixed-mode core-shell column in RP/cation-exchange mode. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of torbutrol in various sample matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | 65% ACN with 0.1% of H3PO4 |
Detection: | UV 215 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.6 mL/min |
Analytes
Class of compounds: | Aromatic acid, Aromatic base, Controlled substance, Drug |
Nature of compounds: | Basic, Hydrophilic, Hydrophobic |
Compounds: | Torbutrol, Related Impurities |