Application description
Tramadol is an opioid analgesic. It is used to treat moderate and severe pain. It is often combined with Acetaminophen to improve the efficacy of tramadol in relieving pain. It is converted in the body to Desmethyltramadol. The available dosage forms include liquids, syrups, drops, elixirs, effervescent tablets and powders for mixing with water, capsules, and tablets. This includes extended release formulations, suppositories, compounding powder, and injections. We developed a method for tramadol and related impurities on the Coresep 100 mixed-mode column. Tramadol is a hydrophobic basic compound. Tramadol and related impurities are retained and separated based on reversed-phase and cation-exchange mechanism. The retention time, resolution and peak shape are controlled by the amount of organic, buffer pH and buffer concentration. Various mobile phases can be used with Coresep 100 column to accommodate multiple detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 100A |
| Mobile Phase: |
ACN from 10 to 80% in 12 min / Buffer: AmFm pH 3from 10 to 40 mM in 12 min |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.6 mL/min |
Analytes
| Class of compounds: |
Controlled substance, Drug |
| Nature of compounds: |
Basic, Hydrophobic |
| Compounds: |
Tramadol |
