HPLC Analysis of Drug Tramadol on Coresep 100 Mixed-Mode Column

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 65% ACN with 0.1% of H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
Class of compounds: Controlled substance, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Tramadol