HPLC Analysis of Drug Vicodin on Heritage MA Mixed-Mode Column

Application description

Codeine/paracetamol, also known as codeine/acetaminophen or hydrocodone/APAP is the combination of an opioid pain medication, codeine, with paracetamol (acetaminophen). It is used as a prescription drug to relieve moderate to severe pain. It exists in tablet, elixir, and solution forms in various strengths for oral administration. Vicodin is Schedule II controlled substance. Codeine diversion and recreational use has escalated in recent years due to its opioid effects. Vicodin was analyzed with isocratic conditions to provide fast, reliable and robust method for analysis of both components of the drug combination - codeine and acetaminophen. Compounds are retained and separated on Heritage MA HPLC column in reversed-phase and cation-exclusion mechanisms. Retention time, selectivity and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection).

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and cation-exclusion
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 5% ACN with 50 mM AmAc pH 4
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic base, Aromatic compound, Controlled substance, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral
Compounds: Codeine, Acetaminophen