HPLC Analysis of Drugs Octopamine and Synephrine on Core-Shell Mixed-Mode Coresep 100 Column

Application description

Octopamine is an endogenous biogenic amine similar to norepinephrine. Octopamine can serve as a neurotransmitter, neurohormone and neuromodulator. It is hydrophilic and basic in nature. Synephrine is an alkaloid and a biogenic amine similar to norepinephrine. It is hydrophilic and basic in nature. Both of these compounds are hydrophilic and have poor retention on traditional reversed-phase columns unless an ion-pairing reagent is used. The retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust and reproducible, and can be used for analysis of both octopamine and synephrine in various matrices (reaction mixtures, formulations, biofluids). It does not require ion-pairing reagents (IPC). Various mobile phases can be used with the Coresep 100 column to accommodate multiple detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: MeCN 30% with 30 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Amines, Aromatic base, Drug, Neurotransmitter
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Octopamine, Synephrine