HPLC Analysis of Eleven Compounds on Amaze C18 SPF Column. Effect of Acetonitrile

Conditions of Experiment
Column: Amaze C18
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: ACN/Water/Ammonium phosphate/phosphoric acid
Detection: 215 nm
Sample: 0.2-0.4 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Antibiotic, Aromatic base, Aromatic compound, Drug, Organic compound, Organic solvent, Preservative
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds: Ciprofloxacin, Pefloxacin, Propranolol, Diphenhydramine, Methylparaben, Amitriptyline, Ethylparaben, Dimethyl phthalate, Sertraline, Propylparaben, Toluene