HPLC Analysis of Epinephrine and Phenylephrine on Heritage C18 Column According to US Pharmacopeia

HPLC Analysis of Epinephrine and Phenylephrine on Heritage C18 Column According to US Pharmacopeia chromatogram
Heritage С18 PURCHASE LEARN ABOUT Heritage С18
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: Water/MeOH ( 50/50) with 0.1% 1-octanesulfonic acid adjust to pH 3.0 with H3PO4
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Catecholamine, Drug, Neurotransmitter
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Epinephrine, Phenylephrine