HPLC Analysis of Epinephrine and Phenylephrine on Heritage C18 Column According to US Pharmacopeia

Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: Water/MeOH ( 50/50) with 0.1% 1-octanesulfonic acid adjust to pH 3.0 with H3PO4
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Class of compounds: Catecholamine, Drug, Neurotransmitter
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Epinephrine, Phenylephrine