HPLC Analysis of Ethanolamines

Application description

Ethanolamines are commonly used basic compounds which often act as surfactants and cleansing agents. Diethanolamine for example, is used for inhibiting corrosion, industrial gas scrubbing to remove hydrogen sulfide from gas, and used in production of morpholine. Coresep 100 was used to separate monoethanolamine, diethanolamine, triethanolamine, and morpholine, a pH adjusting agent used in the petroleum industry synthesized from diethanolamine. Coresep 100 is a mixed-mode column that retains bases using cation-exchange. Ethanolamines are volatile and have no UV activity. A refraction index detector was used to analyze ethanolamines.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: 2% of ACN with 5 mM AmAc pH 3.0
Detection: RI
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Ethanolamine
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Ethanolamine, Diethanolamine, Triethanolamine, Morpholine