HPLC Analysis of Ethylenediaminetetraacetic acid (EDTA) on Amaze TH Mixed-Mode Column

Application description

EDTA is polar zwitter-ionic compound with very strong chelating properties. It is used in textile, cosmetics, pulp and paper industries as well as chemical and pharmaceutical applications. Ethylenediaminetetraacetic acid is widely used as preservative and additive to drug compositions and formulations. Due to a very polar nature of EDTA it is impossible to retain and analyze it without the use of ion-pairing reagent or alternative separation techniques like HILIC and ion-exchange. In our method, EDTA was analyzed in HILIC/ion-exchange mode on Amaze TH HILIC mixed-mode HPLC column. Method is robust and reproducible and can be used to quantify EDTA in various products. Retention time is controlled by amount of buffer, buffer pH and amount of ACN.

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6 x 50 mm, 5 um, 100A
Mobile Phase: 75% ACN with 25 mM AmFm pH 3.8
Detection: ELSD
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Organic acid, Preservative
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Ethylenediaminetetraacetic acid