HPLC Analysis of Excedrin Pain Medication on Reversed-Phase Aromatic Phases

Conditions of Experiment
Column: Amaze C18
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 25% ACN with 50 mM ammonium phosphate and 0.1% phosphoric acid
Detection: 220 nm
Sample: 0.3-0.5 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Aromatic compound, Drug, Stimulant, Xanthine
Nature of compounds: Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Acetaminophen, Caffeine, Aspirin