HPLC Analysis of Extract of CBN Patch

Application description

With legalization of hemp production as well as medical and recreational marijuana, there is a need to develop universal approaches for the analysis of cannabinoids. Cannabinol is one of the 13 active cannabinoids occurring in marijuana and hemp plants. Cannabinol is mildly psychoactive. There are only traces of CBN in marijuana plants. Cannabinol forms from tetrahydrocannabinolic acid (THCA) during the curing and aging process. If cannabis is exposed to oxygen and a UV light, THCA will first convert to cannabinolic acid (CBNA) and then decarboxylate to CBN. CBN from marijuana plants is used as an additive to food as well as a component of creams and patches. Cannabinol is very hydrophobic in nature and is not soluble in water. It is retained on the Daze 47 HPLC column by reversed-phase mechanism. The retention time is adjusted by the amount of organic component in the mobile phase. This method and column can be used for the analysis of cannabinoids in various cannabis products. Some sample preparation is required for edibles.

Conditions of Experiment
Column: Daze 47
Separation Modes:
Column Dimenstions: 3.0 x 150 mm, 5 um, 100A
Mobile Phase: 70% ACN with 0.1% H2SO4
Detection: UV 275 nm
Flow rate: 1 ml/min
Class of compounds: Aromatic compound, Cannabinoid
Nature of compounds: Hydrophobic, Neutral
Compounds: CBN