HPLC Analysis of Five Main Cannabinoids on Heritage C18 Column

Application description

With legalization of marijuana and marijuana related products in USA, Canada and other countries there is a need for analysis for potency and contaminants in these products. The method for potency of marijuana requires separation and quantification of main components of marijuana. The main components of marijuana are THC, THCA, CBN, CBD, and CBDA. Tetrahydrocannabinolic acid (THCA) is active component of marijuana and a precursor of tetrahydrocannabinol (THC). It is acidic and hydrophobic in nature. It undergoes decarboxylation to from tetrahydrocannabinol (THC). Tetrahydrocannabinol (THC) is one of dozens of cannabinoids identified in cannabis. It is a psychoactive component. It is Schedule II compound. Cannabidiol (CBD) is one of dozens of cannabinoids. The hemp extract contains up to 40% of CBD and is used to produce this pharmaceutically active ingredient of cannabis. It is used as pain medication and medication to treat epilepsy. CBDA is obtained from CBDA by decarboxylation of cannabidiolic acid (CBDA). Fresh extract of marijuana plants contains 95% THCA and 5% CBDA. Cannabinol (CBN) is non-psychoactive compound found only in traces in cannabis. Unlike other cannabinoids it is not derived from cannabigerol (CBG). CBN is not a controlled substance. We have developed an effective separation of CBD, CBDA, CBN, THC and THCA on Heritage C18 HPLC column. Compounds are separated based on reversed-phase mechanism. The method can be used for analysis of cannabinoids in marijuana, marijuana related products, THC and CBD infused edibles, etc.

Conditions of Experiment
Column: Heritage C18
Separation Modes: reversed-phase
Column Dimenstions: 3.2 x 150 mm, 5 um, 100A
Mobile Phase: MeCN 70% with 0.1% phosphoric acid
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.2 mL/min
Class of compounds: Aromatic acid, Aromatic compound, Cannabinoid, Controlled substance, Drug
Nature of compounds: Acidic, Hydrophobic, Neutral
Compounds: CBDA, CBD, CBN, THC, THCA