HPLC Analysis of Five Sugars on Amaze SC Mixed-Mode Column in HILIC Mode

Conditions of Experiment
Column: Amaze SC
Separation Modes: HILIC
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: A: 98% ACN with 0.02% TFA B: 85% ACN with 0.1% TFA from 100% A to 100% B in 12 min and 3 min hold
Detection: Corona CAD
Sample: approx 0.5 mg/ml
Injection: 3 μL
Flow rate: 0.6 ml/min
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Glucose, Maltitol, Sucrose, Lactose