HPLC Analysis of Four Sugars in HILIC Mode on Amaze HD Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: ACN/Water/TFA
Detection: ELSD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Class of compounds: Sugar
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: Fructose, Glucose, Sucrose, Raffinose