HPLC Analysis of Fungicide Tebuconazole on Coresep 100 Mixed-Mode Column
| Conditions of Experiment |
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min with 3 min hold |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.7 mL/min |
|
| Analytes |
1. Methiocarb
|
Class of compounds:
Herbicide, Pesticide |
|
Nature of compounds:
Hydrophobic, Neutral |
Application description
Tebuconazole is triazole fungicide used in agriculture to treat pathogenic fungi. Tebuconazole is hydrophobic and slightly basic. It retains on Coresep 100 column by combination of strong reversed-phase and weak cation-exchange mechanisms. This robust method is compatible with major detection techniques including mass spectrometry.
