HPLC Analysis of Fungicide Tebuconazole on Coresep 100 Mixed-Mode Column
Application description
Tebuconazole is triazole fungicide used in agriculture to treat pathogenic fungi. Tebuconazole is hydrophobic and slightly basic. It retains on Coresep 100 column by combination of strong reversed-phase and weak cation-exchange mechanisms. This robust method is compatible with major detection techniques including mass spectrometry.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min with 3 min hold |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Herbicide, Pesticide |
Nature of compounds: | Hydrophobic, Neutral |
Compounds: | Methiocarb |