HPLC Analysis of Glucose and Lysine on Amaze TH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchnage
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: 65% ACN with 175 mM AmAc pH 5
Detection: ELSD 45°C, RI-compatible
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Sugar, Supplement
Nature of compounds: Hydrophilic, Neutral, Polar, Zwitterionic
Compounds: Glucose, Lysine