HPLC Analysis of Glucose, Gluconic and Glucuronic Acids on Amaze TH Mixed-Mode Column
Conditions of Experiment
| Column: | Amaze TH |
| Separation Modes: | HILIC, anion-exchange, cations-exchange |
| Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
| Mobile Phase: | 80% ACN with 20 mM AmFm pH 3 |
| Detection: | ELSD |
| Sample: | 0.3-0.5 mg/ml |
| Injection: | 3 uL |
| Flow rate: | 1 ml/min |
Analytes
| Class of compounds: | Inorganic cation, Organic acid, Sugar |
| Nature of compounds: | Acidic, Basic, Hydrophilic, Neutral, Polar |
| Compounds: | Glucose, Gluconic acid, Glucuronic acid, Sodium ion |