HPLC Analysis of Glucose, Gluconic and Glucuronic Acids on Amaze TH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, anion-exchange, cations-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD
Sample: 0.3-0.5 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Inorganic cation, Organic acid, Sugar
Nature of compounds: Acidic, Basic, Hydrophilic, Neutral, Polar
Compounds: Glucose, Gluconic acid, Glucuronic acid, Sodium ion