HPLC Analysis of Glycine-Zinc Formulation on Amaze SC in Reversed-Phase and Cation-Exchange Modes

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange and anion-exclusion
Column Dimenstions: 4.6x50 mm, 3 μm, 100A
Mobile Phase: 10% ACN, TFA from 0.05% to 0.25% in 3 min, 5 min hold
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Class of compounds: Amino acid, Inorganic anion, Inorganic cation, Organic acid, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Bromide ion, Glycine, Zinc ion