HPLC Analysis of Glyphosate. Effect of Mobile Phase Composition on Retention Time, Efficiency and Symmetry of Glyphosate

Conditions of Experiment
Column: Amaze HA
Separation Modes: reveresed-phase, anion-exchange
Column Dimenstions: 2.1x100 mm, 3 um, 100A
Mobile Phase: ACN/water/ammonium formate
Detection: Corona CAD
Sample: 0.3 mg/ml
Injection: 2 uL
Flow rate: 0.25 ml/min
Analytes
Class of compounds: Herbicide, Organic acid, Pesticide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Glyphosate