HPLC Analysis of Guanidine and Counterions in in HILIC Ion-Exchange and HIIC Ion-Exclusion Modes

Conditions of Experiment
Column: Amaze TH, Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 85% ACN with 30 mM AmFm pH 3
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Aromatic base, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Nitrate ion, Chloride Ion, Guanidine, Sodium ion