HPLC Analysis of Guanidine and Counterions in in HILIC Ion-Exchange and HIIC Ion-Exclusion Modes
Conditions of Experiment
Column: | Amaze TH, Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exchange, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | 85% ACN with 30 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amines, Aromatic base, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Nitrate ion, Chloride Ion, Guanidine, Sodium ion |