Application description
2,4-D or 2,4-dichlorophenoxyacetic acid is phenoxyacetic herbicide used in the treatment of broadleaf weeds. 2,4-D is one of the most widely used herbicides and defoliant. 2,4-D is manufactured from chloroacetic acid and 2,4-dichlorophenol. 2,4-D is often used in combination with dicamba, mecoprop, and MCPA. 2,4-D was analyzed on core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and anion-exclusion |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.7 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Herbicide, Pesticide |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
2,4-D |
