HPLC Analysis of Herbicide 2,4-DB on Coresep 100 Mixed-Mode Column

Application description

2,4-DB or 4-(2,4-dichlorophenoxy)butyric acid is a selective systemic phenylbutyric herbicide used to control annual and perennial broadleaf weeds. 2,4-DB is acidic and hydrophobic in nature and can be analyzed on Coresep 100 core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by an amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Aromatic acid, Herbicide, Pesticide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: 2,4-DB