HPLC Analysis of Herbicide 2,4-DB on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exclusion
Column Dimenstions: 4.6 x 50 mm
Mobile Phase: 45% ACN with 80 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic acid, Herbicide, Pesticide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: 2,4-DB