HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column

Application description

2,4,5- Trichlorophenoxyacetic acid or 2,4,5-T is chlorophenoxy acetic herbicide used to defoliate broad-leafed plants. It is hydrophobic and acidic in nature and was analyzed on Coresep 100 mixed-mode column in reversed-phase and anion-exchange modes. Retention time can be adjusted by the amount of ACN, buffer concentration and buffer pH. This method demonstrates good retention control and perfect peak shape for this acidic and hydrophobic analyte. The method is compatible with major detection techniques (UV, ELSD, CAD, RI, and MS).

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Aromatic acid, Herbicide, Pesticide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: 2,4,5-T