HPLC Analysis of Herbicide Bentazon on Coresep 100 Mixed-Mode Column

Application description

Bentazon is a thiadiazine herbicide. It is hydrophobic and neutral in nature. Bentazon was retained and analyzed on core-shell mixed-mode Coresep 100 column. The compound is retained by the reversed-phase mechanism. The retention time can be adjusted by changing the amount of the acetonitrile in the mobile phase. This method can be used for analysis of neutral and ionizable herbicides and pesticides in various matrices like soil, wastewaters, crops, etc. The method is compatible with mass spectrometry and is robust with good peak shape and retention control.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Aromatic compound, Herbicide
Nature of compounds: Hydrophobic, Neutral
Compounds: Bentazon