HPLC Analysis of Herbicide Dichlorprop on Coresep 100 Mixed-Mode Column
Application description
Dichlorprop is chlorophenoxy herbicide which is used to kill perennial broadleaf weeds. Dichlorprop is hydrophobic and acidic in nature and is sold as a salt. Dichlorprop was analyzed in reversed-phase anion-exclusion mode on Coresep 100 core-shell mixed-mode column. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles. The method and Coresep 100 column can be used for analysis of various herbicides in complex sample matrices. The approach is compatible with major detection techniques (UV, ELSD, CAD, RI, and MS).
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and anion-exclusion |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Aromatic acid, Herbicide |
Nature of compounds: | Acidic, Hydrophobic |
Compounds: | Dichlorprop |