HPLC Analysis of Herbicide MCPA on Coresep 100 Mixed-Mode Column

HPLC Analysis of Herbicide MCPA on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
1. MCPA

Class of compounds: Aromatic acid, Herbicide
Nature of compounds: Acidic, Hydrophilic, Polar

Application description

MCPA or (2-methyl-4-chlorophenoxyacetic acid is a powerful and selective phenoxy herbicide. It is used to control broadleaf weeds. It is also used in the chemical industry due to the ability to form conjugated complexes with metals. MCPA is hydrophobic and acidic and retains on Coresep 100 mixed-mode column by the combination of reversed-phase and anion-exclusion mechanisms. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles.

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