HPLC Analysis of Herbicide MCPB on Coresep 100 Mixed-Mode Column

Application description

MCPB, 2,4-MCPB, 4-(4-chloro-o-tolyloxy)butyric acid, or 4-(4-chloro-2-methylphenoxy) butanoic acid is phenoxybutyric herbicide. US EPA established striked levels of MCPB permitted in crops. MCPB is hydrophobic and acidic in nature. It was analyzed in reversed-phase anion-exclusion mode on Coresep 100 mixed-mode HPLC column. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Cores-shell mixed-mode columns are a versatile tool for analysis of polar, hydrophobic and ionic compounds. This method is compatible with mass spec. The approach can be used for analysis of MCPB in soil, crops and food products.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Aromatic acid, Herbicide
Nature of compounds: Acidic, Hydrophobic, Polar
Compounds: MCPB