HPLC Analysis of Herbicide Mecoprop on Coresep 100 Mixed-Mode Column
Application description
Mecoprop or methylchlorophenoxypropionic acid (MCPP) is common use herbicide used to control broadleaf weeds. It is often used in combination with 2,4-D, dicamba, and MCPA. Mecoprop is a mixture of two stereoisomers. The compound is acidic and hydrophobic in nature. Mecoprop was analyzed on core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | |
Nature of compounds: | |
Compounds: |