HPLC Analysis of Herbicide Mecoprop on Coresep 100 Mixed-Mode Column

Application description

Mecoprop or methylchlorophenoxypropionic acid (MCPP) is common use herbicide used to control broadleaf weeds. It is often used in combination with 2,4-D, dicamba, and MCPA. Mecoprop is a mixture of two stereoisomers. The compound is acidic and hydrophobic in nature. Mecoprop was analyzed on core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds:
Nature of compounds: