HPLC Analysis of Herbicide Triclopyr on Coresep 100 Mixed-Mode Column

Application description

Triclopyr (3,5,6-Trichloro-2-pyridinyloxyacetic acid) is herbicide. Triclopyr is used to control broadleaf weeds while leaving grasses and conifers unaffected or to control rust diseases on crops. Triclopyr is low hydrophobicity zwitterionic compound which has carboxylic acid fragment and pyridine ring. Triclopyr was analyzed on Coresep 100 in mixed-mode chromatography in reversed-phase cation-exchange mode. Ionization state of carboxylic acid in this herbicide can be suppressed by using low pH mobile phases. This enhances cation-exchange interaction coming from stationary phase. Triclopyr is analyzed without ion-pairing reagent in the mobile phase. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of triclopyr in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm
Mobile Phase: 30% ACN with 30 mM AmFm pH 3
Detection: UV 235 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Fungicide, Pesticide, Pyridine
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Triclopyr (3,5,6-Trichloro-2-pyridinyloxyacetic acid)