HPLC Analysis of Herbicide Trifluralin on Coresep 100 Mixed-Mode Column

HPLC Analysis of Herbicide Trifluralin on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm
Mobile Phase: 50% ACN with 30 mM AmFm pH 3
Detection: UV 235 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
1. Trifluralin

Class of compounds: Herbicide, Pesticide
Nature of compounds: Basic, Hydrophobic

Application description

Trifluralin is a commonly used pre-emergence herbicide. Trifluralin is generally applied to the soil to provide control of a variety of annual grass and broadleaf weed species. It inhibits root development by interrupting mitosis, and thus can control weeds as they germinate. Trifluralin is hydrophobic compound. It has some weak basic properties due to the presence of aniline fragment in the molecule. Trifluralin was analyzed by mixed-mode chromatography on Coresep 100 mixed-mode reversed-phase cation-exchange column. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of trifluralin in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

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