HPLC Analysis of Herbicides Mepiquat and Ethephon on Amaze HILIC Mixed-Mode Columns

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 80% ACN with 10 mM AmFm pH 3
Detection: ELSD
Sample: 0.3-0.6 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Herbicide, Inorganic anion, Organic acid, Quaternary amine
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Chloride Ion, Ethephon, Mepiquat