HPLC Analysis of Histidine and Related Impurities

Conditions of Experiment
Column: Coresep 100
Separation Modes:
Column Dimenstions: 4.6 x 150 mm, 2.7 um, 90A
Mobile Phase: ACN from 2% to 20%, Na2HPO4 pH 6 from 10 mM to 50 mM in 12 min
Detection: UV 210 nm
Sample:
Injection:
Flow rate: 1 mL/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Alanine, Histidine, Lysine, Arginine