HPLC Analysis of Hydrazine on Coresep 100 Mixed-Mode Column

Application description

Hydrazine is a highly polar and toxic compound which is used as a precursor to pesticides and pharmaceuticals. It is used in coupling reactions as well as hetero atom-containing rings such as pyrazoles and pyridazines. It is also used as a part of agricultural formulations. Due to its toxicity, a rapid and accurate determination of hydrazine in various products and matrices is required. Hydrazine, due to its polar nature, has no retention on reversed-phase columns. We have developed a fast and robust HPLC-ELSD method for the determination of hydrazine. It is retained on the Coresep 100 mixed-mode cation-exchange column by cation-exchange mechanism. Various mobile phases can be used to facilitate the retention of hydrazine. The replacement of trifluoroacetic acid with formic acid or ammonium formate makes this method MS-compatible.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: aqeous TFA
Detection: ELSD, 40°C
Sample: 0.5 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Class of compounds: Amines, Organic compound
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Hydrazine