HPLC Analysis of Hydrophilic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes

Application description

Mixture of hydrophilic acidic compounds are separated in reversed-phase anion-exchange mode on core-shell mixed-mode column. Method is robust, reliable and fast and can be used for analysis of underivatized acids without ion-pairing reagent.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 50% ACN with 50 mM sodium phosphate (monobasic) pH 3
Detection: UV 230 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 0.5 mg/ml
Class of compounds: Aromatic acid, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Benzoic acid, Maleic acid, Fumaric acid