Application description
Mixture of hydrophilic acidic compounds are separated in reversed-phase anion-exchange mode on core-shell mixed-mode column. Method is robust, reliable and fast and can be used for analysis of underivatized acids without ion-pairing reagent.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase and anion-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
50% ACN with 50 mM sodium phosphate (monobasic) pH 3 |
| Detection: |
UV 230 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.5 mg/ml |
Analytes
| Class of compounds: |
Aromatic acid, Organic acid |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
Benzoic acid, Maleic acid, Fumaric acid |
