HPLC Analysis of Illicit Drug Heroin on Coresep 100 Mixed-Mode Column

HPLC Analysis of Illicit Drug Heroine on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 60% CAN with 30 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 mL/min
Analytes
1. Heroin

Class of compounds: Controlled substance, Illegal drug
Nature of compounds: Basic, Hydrophobic

Application description

Heroin is an opioid recreational illegal drug. It is Schedule I controlled substance. Heroin is hydrophobic and basic in nature. It is retained on mixed-mode Coresep 100 HPLC column by combination of strong hydrophobic and strong cation-exchange mechanisms. This method is robust, fast and reliable with good retention and peak shape for highly hydrophobic and basic molecule. Retention time is controlled by the amount of acetonitrile, buffer concentration, and buffer pH. The method can be applied to the analysis of other illegal drugs in biofluids and formulations. The method is isocratic and fully compatible with LC/MS.

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