HPLC Analysis of Insecticide Carbaryl on Coresep 100 Mixed-Mode Column

Application description

Carbaryl is carbamate insecticide. Carbaryl is a cholinesterase inhibitor which is toxic to humans. Strict guidelines are established for levels on carbaryl in crops, soils, and other agricultural matrices. Carbaryl is hydrophobic in nature and retains on Coresep 100 mixed-mode column by reversed-phase mechanism. The compound is well retained. Retention time is controlled by the amount of the ACN in the mobile phase. This method can be used for analysis of various pesticides, herbicides, fungicides, and insecticides. Core-shell mixed-mode columns offer a combination of unique selectivity and high efficiency while using regular equipment.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Herbicide, Insecticide
Nature of compounds: Hydrophobic, Neutral
Compounds: Carbaryl